RNA
Part:BBa_K4608003:Design
Designed by: Albert Nitu Group: iGEM23_McGill (2023-10-12)
Empty guide RNA scaffold for Cas7-11
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For designing this sequence, we had to consider an easy way to design the empty region of the gRNA so that researchers could easily and scalably clone in any spacer sequence they wish into their gRNA expression vector. We settled on using Golden Gate assembly for this, as we foudn it has the highest efficiency.
Source
The guide RNA scaffold originates from the original bacterium that expresses the Craspase system, Desulfonema ishimotonii.