RNA

Part:BBa_K4608003:Design

Designed by: Albert Nitu   Group: iGEM23_McGill   (2023-10-12)


Empty guide RNA scaffold for Cas7-11


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For designing this sequence, we had to consider an easy way to design the empty region of the gRNA so that researchers could easily and scalably clone in any spacer sequence they wish into their gRNA expression vector. We settled on using Golden Gate assembly for this, as we foudn it has the highest efficiency.


Source

The guide RNA scaffold originates from the original bacterium that expresses the Craspase system, Desulfonema ishimotonii.

References